譜析（上海）生物科技有限公司優(yōu)勢供應(yīng)：E. coli Gyrase Cleavage Assay Kits
2019/10/11 14:48:56
這導(dǎo)致細(xì)胞死亡，這是喹諾酮類藥物，如納立二酸和環(huán)丙沙星作用背后的機(jī)制。卵裂分析在確定潛在藥物是否通過這一機(jī)制起作用時特別有用。這些試劑盒是專門為裂解反應(yīng)設(shè)計的。除了酶和線性化的pBR322標(biāo)記外，它們還包含超螺旋的pBR322 DNA底物、DNA裂解反應(yīng)所需的分析和稀釋緩沖液。解理特定的酶可單獨(dú)要求。
Some drugs interrupt the DNA breakage-reunion step of the gyrase reaction. This leads to cell death and it is the mechanism behind the action of the quinolones such as nalidixic acid and ciprofloxacin. Cleavage assays are particularly useful in determining if a potential drug acts by this mechanism.These kits are designed specifically for cleavage reactions. They contain the supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to the enzyme and linearised pBR322 marker.
Cleavage specific enzyme available separately on request.
優(yōu)勢供應(yīng)產(chǎn)品：
| Cat No. | Product | Product description |
| GCK001 | E.coli Gyrase Cleavage Kit 1 |
100 U of E. coli gyrase and 50 μg of supercoiled DNA |
| GCK002 | E.coli Gyrase Cleavage Kit 2 |
500 U of E. coli gyrase and 250 μg of supercoiled DNA |
| GCK003 | E.coli Gyrase Cleavage Kit 3 |
1000 U of E. coli gyrase and 500 μg of supercoiled DNA |
| GCK004 | E.coli Gyrase Cleavage Kit 4 |
2000 U of E. coli gyrase and 1 mg of supercoiled DNA |
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